Queen + Adam Lambert dedicated their Isle of Wight Festival set to those killed and injured in a mass shooting at an Orlando gay nightclub on Sunday.

Forty-nine people died and dozens were injured when a lone gunman opened fire in the Pulse nightclub.

According to several reports, the group played a two-hour set.

Lambert, who has been touring with original Queen members Brian May and Roger Taylor for several years, dedicated Who Wants To Live Forever to the victims of Sunday's attack.

“This song is dedicated to those who lost their lives last night in Orlando, Florida, and anyone who has been a victim of senseless violence or hatred,” Lambert told the crowd.

Footage of Queen frontman Freddie Mercury, who died from AIDS complications in 1991, played throughout the concert.

“Thank you to the rock-n-roll royalty that I get to share a stage with, Brian May and Roger Taylor,” Lambert said. “This is one of the biggest honors in my life. There will always be, for all time, one Freddie.”